Human Tropomyosin Receptor Kinase B (TrkB) ELISA Kit

Cat.#:

JL54315

Regulatory Status:

RUO

Alternative:

GP145-TrkB; NTRK2; BDNF/NT-3 growth factors receptor; Neurotrophic Tyrosine Kinase Receptor Type 2; Tropomyosin-related kinase B; TrkB tyrosine kinase

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.06ng/mL

Specificity:

This kit recognizes Human TrkB in samples. No significant cross-reactivity or interference between Human TrkB and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates and other biological fluids

Assay Procedure:

Research Area:

Neuroscience

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| TrkB

Tropomyosin receptor kinase B (TrkB),also known as tyrosine receptor kinase B, or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 is a protein that encoded by the NTRK2 gene.TrkB is a receptor for brain-derived neurotrophic factor (BDNF).Tropomyosin receptor kinase B is the high affinity catalytic receptor for several "neurotrophins", which are small protein growth factors that induce the survival and differentiation of distinct cell populations. The neurotrophins that activate TrkB are: BDNF (Brain Derived Neurotrophic Factor), neurotrophin-4 (NT-4), and neurotrophin-3 (NT-3).As such, TrkB mediates the multiple effects of these neurotrophic factors, which includes neuronal differentiation and survival.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human TrkB spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	92-105	101
Cell Lysates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human TrkB and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	88-96	90-110
1 : 2	Average (%)	93	96
1 : 4	Range (%)	87-108	105-115
1 : 4	Average (%)	98	108